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Image Search Results
Journal: International Journal of Alzheimer's Disease
Article Title: Intracellular APP Domain Regulates Serine-Palmitoyl-CoA Transferase Expression and Is Affected in Alzheimer's Disease
doi: 10.4061/2011/695413
Figure Lengend Snippet: SPTLC2 expression is reduced in the presence of functional AICD. (a) Mouse embryonic fibroblasts expressing an APP construct lacking the last 15 amino acids (aa) and therefore a functional AICD domain (MEF APP∆CT15) show increased SPT expression and activity compared to control fibroblasts (MEF WT). (b) MEF APP∆CT15 cells incubated with functional AICD peptide show compared to MEF APP∆CT15 cells incubated with solvent control decreased SPTLC2 expression. (c) MEF APP∆CT15 cells incubated with A β peptides and solvent control showed no difference in SPTLC2 expression. (d) SPTLC2 expression in Fe65 knock-down SH-SY5Y cells is increased.
Article Snippet: To determine the effect of A β 40 (10 ng/mL) and A β 42 (1 ng/mL) (B. Penke, Szeged, Hungary) or
Techniques: Expressing, Functional Assay, Construct, Activity Assay, Control, Incubation, Solvent, Knockdown